| First Author | Orhon I | Year | 2016 |
| Journal | Nat Cell Biol | Volume | 18 |
| Issue | 6 | Pages | 657-67 |
| PubMed ID | 27214279 | Mgi Jnum | J:236815 |
| Mgi Id | MGI:5807315 | Doi | 10.1038/ncb3360 |
| Citation | Orhon I, et al. (2016) Primary-cilium-dependent autophagy controls epithelial cell volume in response to fluid flow. Nat Cell Biol 18(6):657-67 |
| abstractText | Autophagy is an adaptation mechanism that is vital for cellular homeostasis in response to various stress conditions. Previous reports indicate that there is a functional interaction between the primary cilium (PC) and autophagy. The PC, a microtubule-based structure present at the surface of numerous cell types, is a mechanical sensor. Here we show that autophagy induced by fluid flow regulates kidney epithelial cell volume in vitro and in vivo. PC ablation blocked autophagy induction and cell-volume regulation. In addition, inhibition of autophagy in ciliated cells impaired the flow-dependent regulation of cell volume. PC-dependent autophagy can be triggered either by mTOR inhibition or a mechanism dependent on the polycystin 2 channel. Only the LKB1-AMPK-mTOR signalling pathway was required for the flow-dependent regulation of cell volume by autophagy. These findings suggest that therapies regulating autophagy should be considered in developing treatments for PC-related diseases. |
