| Primary Identifier | MGI:2386593 | Allele Type | Targeted |
| Gene | Vegfa | Transmission | Germline |
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The floxed neo cassette was excised from Vegfatm3Pec by the in vivo expression of cre recombinase, leaving the isoform specific cDNA replacement intact. The resultant allele lacks exon 6 as well as splice sites and thereby produces a single isoform with intermediate solubility and receptor binding properties. The exclusive presence of this isoform was verified by quatitative RT-PCR. |