| First Author | Reddy GR | Year | 2022 |
| Journal | iScience | Volume | 25 |
| Issue | 1 | Pages | 103693 |
| PubMed ID | 35036877 | Mgi Jnum | J:328681 |
| Mgi Id | MGI:6856471 | Doi | 10.1016/j.isci.2021.103693 |
| Citation | Reddy GR, et al. (2022) Deciphering cellular signals in adult mouse sinoatrial node cells. iScience 25(1):103693 |
| abstractText | Sinoatrial node (SAN) cells are the pacemakers of the heart. This study describes a method for culturing and infection of adult mouse SAN cells with FRET-based biosensors that can be exploited to examine signaling events. SAN cells cultured in media with blebbistatin or (S)-nitro-blebbistatin retain their morphology, protein distribution, action potential (AP) waveform, and cAMP dynamics for at least 40 h. SAN cells expressing targeted cAMP sensors show distinct beta-adrenergic-mediated cAMP pools. Cyclic GMP, protein kinase A, Ca(2+)/CaM kinase II, and protein kinase D in SAN cells also show unique dynamics to different stimuli. Heart failure SAN cells show a decrease in cAMP and cGMP levels. In summary, a reliable method for maintaining adult mouse SAN cells in culture is presented, which facilitates studies of signaling networks and regulatory mechanisms during physiological and pathological conditions. |
