| Primary Identifier | MGI:2386202 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Mesp2 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | true | Is Wild Type | false |
| description | Chimera analysis in which double homozygous Mesp1tm3Ysa and Mesp2tm3(cre)Ysa mutant embryos were aggregated with wild-type embryos show that embryos develop normally, however double knock-out cells are scarcely seen in the anterior-cephalic and heart mesoderm, but they do contribute to the formation of somites, notochord, and gut, and the atrial appendage is exclusively composed of the mutant cells (J:62984). |
| molecularNote | The entire Mesp2 gene was replaced with an in-frame cre gene and a PGK-puro cassette by homologous recombination into an ES cell line containing a disrupted Mesp1 allele (Mesp1tm3Ysa). This allele expresses Cre recombinase under the control of the Mesp2 promoter. |
