| Primary Identifier | MGI:2386281 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rad51d |
| Transmission | Germline | Strain of Origin | (C57BL/6J x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene was disrupted by replacement of exons 4-6 with a neomycin resistance cassette via homologous recombination. The targeted mutation results in the loss of a conserved DNA-binding motif called the Walker box, and also causes a frameshift. Homozygous mutant embryos were detected by PCR genotype analysis up to day E10.5. The targeted allele was confirmed by RT-PCR analysis of E8.5 embryos using primers flanking the deleted region. No RT-PCR products were detected. |
