| Primary Identifier | MGI:2386327 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Igf1r |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 was flanked by a floxed neo cassette inserted into intron 2 and a single loxP site in intron 3. Several aberrant transcripts involving the splicing of exon 2 to cryptic slice sites in the neo transgene were identified by RT-PCR and sequence analyses. Further analysis indicated that the readthrough of the neo transgene will result in functional ablation due to truncation or the inclusion of a random neo fragment. Mice carrying this allele were crossed with transgenic mice expressing cre recombinase via the EIIa promoter to generate Igf1rtm1.1Mhz and Igf1rtm1.2Mhz. |
