| First Author | Shinozuka T | Year | 2024 |
| Journal | Dev Dyn | Volume | 253 |
| Issue | 4 | Pages | 435-446 |
| PubMed ID | 37767857 | Mgi Jnum | J:359121 |
| Mgi Id | MGI:7623886 | Doi | 10.1002/dvdy.659 |
| Citation | Shinozuka T, et al. (2024) Rspo1 and Rspo3 are required for sensory lineage neural crest formation in mouse embryos. Dev Dyn 253(4):435-446 |
| abstractText | BACKGROUND: R-spondins (Rspos) are secreted proteins that modulate Wnt/beta-catenin signaling. At the early stages of spinal cord development, Wnts (Wnt1, Wnt3a) and Rspos (Rspo1, Rspo3) are co-expressed in the roof plate, suggesting that Rspos are involved in development of dorsal spinal cord and neural crest cells in cooperation with Wnt ligands. RESULTS: Here, we found that Rspo1 and Rspo3, as well as Wnt1 and Wnt3a, maintained roof-plate-specific expression until late embryonic stages. Rspo1- and Rspo3-double-knock-out (dKO) embryos partially exhibited the phenotype of Wnt1 and Wnt3a dKO embryos. While the number of Ngn2-positive sensory lineage neural crest cells is reduced in Rspo-dKO embryos, development of dorsal spinal cord, including its size and dorso-ventral patterning in early development, elongation of the roof plate, and proliferation of ependymal cells, proceeded normally. Consistent with these slight defects, Wnt/beta-catenin signaling was not obviously changed in developing spinal cord of dKO embryos. CONCLUSIONS: Our results show that Rspo1 and Rspo3 are dispensable for most developmental processes involving roof plate-derived Wnt ligands, except for specification of a subtype of neural crest cells. Thus, Rspos may modulate Wnt/beta-catenin signaling in a context-dependent manner. |
