| Primary Identifier | MGI:2387553 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Vcam1 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The proximal promoter and exons 1 and 2 were flanked by single loxP sites inserted via homologous recombination. The targeting construct also included a neomycin selection cassette upstream of the promoter elements within the floxed region. Northern blot analysis showed that the inserted loxP sites and neo gene did not interfere with gene expression as the normal levels of both known transcripts were transcribed from the floxed allele. |
