| First Author | Nakamura Y | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 3 | Pages | e91525 |
| PubMed ID | 24621811 | Mgi Jnum | J:215008 |
| Mgi Id | MGI:5604346 | Doi | 10.1371/journal.pone.0091525 |
| Citation | Nakamura Y, et al. (2014) Targeting SUR1/Abcc8-type neuroendocrine KATP channels in pancreatic islet cells. PLoS One 9(3):e91525 |
| abstractText | ATP-sensitive K+ (KATP) channels play a regulatory role in hormone-secreting pancreatic islet alpha-, beta- and delta-cells. Targeted channel deletion would assist analysis and dissection of the intraislet regulatory network. Toward this end Abcc8/Sur1 flox mice were generated and tested by crossing with glucagon-(GCG)-cre mice to target alpha-cell KATP channels selectively. Agonist resistance was used to quantify the percent of alpha-cells lacking channels. 41% of Sur1(loxP/loxP);GCG-cre+ and approximately 64% of Sur1(loxP/-);GCG-cre+ alpha-cells lacked KATP channels, while approximately 65% of alpha-cells expressed enhanced yellow fluorescent protein (EYFP) in ROSA-EYFP/GCG-cre matings. The results are consistent with a stochastic two-recombination event mechanism and a requirement that both floxed alleles are deleted. |
