| Primary Identifier | MGI:2387818 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tsix |
| Transmission | Germline | Strain of Origin | 129 X (M. m. castaneus x 129)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = 16.7, a stable 40XX subclone of ES cell line EL16. 16.7 cells carry one X chromosome from 129 and the other from M. m. castaneus. Southern blot analysis showed the 129 chromosome to be targeted. |
| molecularNote | A floxed neo cassette replaced 3.7 kb of sequence encompassing a CpG island, transcriptional start sites, and the putative promoter. Absence of a 129-specific MnlI restriction pattern indicated that the 129-derived X chromosome was targeted. Expression analysis showed a significant reduction in, if not a complete ablation of, transcription from the targeted allele, though transcript was shown to be produced from the normal X on the untargeted chromosome. Residual transcription was only detected by increased PCR cycles, though it was not clear if this weak transcription was due to minor and/or cryptic promoters or rather to overcylcing conditions. No residual transcription was detected by RNA FISH. |
