| First Author | Bender MA | Year | 2000 |
| Journal | Mol Cell | Volume | 5 |
| Issue | 2 | Pages | 387-93 |
| PubMed ID | 10882079 | Mgi Jnum | J:60714 |
| Mgi Id | MGI:1353825 | Doi | 10.1016/s1097-2765(00)80433-5 |
| Citation | Bender MA, et al. (2000) Beta-globin gene switching and DNase I sensitivity of the endogenous beta-globin locus in mice do not require the locus control region. Mol Cell 5(2):387-93 |
| abstractText | We have generated mice with a targeted deletion of the beta-globin locus control region (LCR). Mice homozygous for the deletion die early in embryogenesis but can be rescued with a YAC containing the human beta-globin locus. After germline passage, deletion of the LCR leads to a severe reduction in expression of all mouse beta-like globin genes, but no alteration in the developmental specificity of expression. Furthermore, a DNase I-sensitive 'open' chromatin conformation of the locus is established and maintained. Thus, the dominant role of the LCR in the native locus is to confer high-level transcription, and elements elsewhere in the locus are sufficient to establish and maintain an open conformation and to confer developmentally regulated globin gene expression. |
