| Primary Identifier | MGI:2386975 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(LPV-TAg1135)11Tvd |
| Strain of Origin | (C57BL/6J x DBA/2J)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Seventeen founders generated: 10, 11, 12, 13, 14, 16, 18, 19, 20, 21, 22, 24, 27, 28, 34, 36, and 37. Line 11 continued. |
| molecularNote | A mutant SV40 T-antigen sequence deleted for amino acids 17-27 and the small t-specific splice donor site was used as the transgene. As a result the large T-antigen should be expressed, but not the small T-antigen. The mutant transgene was fused to the lymphotrophic papovavirus (LPV) enhancer and early promoter to target expression to B- and T-lymphocytes and choroid plexus epithelium. The mutant protein retains the ability to bind RB1, RBL1, and TRP53. Regulatory sequences direct expression of the transgene in B- and T-lymphocytes and choroid plexus epithelium. Western blot analysis of transgenic animals confirmed expression of the transgene in the thymus and spleen. |
