| First Author | Evnouchidou I | Year | 2020 |
| Journal | Nat Commun | Volume | 11 |
| Issue | 1 | Pages | 2779 |
| PubMed ID | 32487999 | Mgi Jnum | J:292266 |
| Mgi Id | MGI:6447715 | Doi | 10.1038/s41467-020-16471-7 |
| Citation | Evnouchidou I, et al. (2020) IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses. Nat Commun 11(1):2779 |
| abstractText | T cell receptor (TCR) activation is modulated by mechanisms such as TCR endocytosis, which is thought to terminate TCR signalling. Here we show that, upon internalization, TCR continues to signal from a set of specialized endosomes that are crucial for T cell functions. Mechanistically, TCR ligation leads to clathrin-mediated internalization of the TCR-CD3zeta complex, while maintaining CD3zeta signalling, in endosomal vesicles that contain the insulin responsive aminopeptidase (IRAP) and the SNARE protein Syntaxin 6. Destabilization of this compartment through IRAP deletion enhances plasma membrane expression of the TCR-CD3zeta complex, yet compromises overall CD3zeta signalling; moreover, the integrity of this compartment is also crucial for T cell activation and survival after suboptimal TCR activation, as mice engineered with a T cell-specific deletion of IRAP fail to develop efficient polyclonal anti-tumour responses. Our results thus reveal a previously unappreciated function of IRAP-dependent endosomal TCR signalling in T cell activation. |
