| First Author | Bergqvist I | Year | 2000 |
| Journal | Eur J Immunol | Volume | 30 |
| Issue | 10 | Pages | 2857-63 |
| PubMed ID | 11069067 | Mgi Jnum | J:65236 |
| Mgi Id | MGI:1913232 | Doi | 10.1002/1521-4141(200010)30:10<2857::AID-IMMU2857>3.0.CO;2-G |
| Citation | Bergqvist I, et al. (2000) The basic helix-loop-helix transcription factor E2-2 is involved in T lymphocyte development. Eur J Immunol 30(10):2857-63 |
| abstractText | E2A, HEB and E2-2 genes encode a group of basic helix-loop-helix (bHLH) transcription factors that are structurally and functionally similar. Deletion of the genes encoding either of these proteins leads to early lethality and a block in B lymphocyte development. Evidence for a function in T lymphocyte development has, however, only been reported for E2A and HEB. To further elucidate the role of E2-2 at developmental stages that have proven difficult to study due to the early lethality phenotype of mice defective in E2-2, we generated and analyzed mice conditionally mutated in the E2-2 gene. These mice are mosaic with respect to E2-2 expression, consisting of cells with either one functional and one null mutated E2-2 allele or two null mutated alleles. Using this experimental model, we find that cells with a homozygous null mutated E2-2 gene are under-represented in B lymphocyte as well as T lymphocyte cell lineages as compared to other hematopoietic or non-hematopoietic cell lineages. Our data suggests that E2-2 deficiency leads to a partial block in both B and T lymphocyte development. The block in T cell development appears to occur at an early stage in differentiation, since skewing in the mosaicism is observed already in CD4+8+ double-positive thymocytes. |
