| First Author | Oliveira KJ | Year | 2014 |
| Journal | Regul Pept | Volume | 194-195 |
| Pages | 30-5 | PubMed ID | 25454367 |
| Mgi Jnum | J:301862 | Mgi Id | MGI:6507242 |
| Doi | 10.1016/j.regpep.2014.10.002 | Citation | Oliveira KJ, et al. (2014) Hypothalamic-pituitary thyroid axis alterations in female mice with deletion of the neuromedin B receptor gene. Regul Pept 194-195:30-5 |
| abstractText | Neuromedin B, a peptide highly expressed at the pituitary, has been shown to act as autocrine/paracrine inhibitor of thyrotropin (TSH) release. Here we studied the thyroid axis of adult female mice lacking neuromedin B receptor (NBR-KO), compared to wild type (WT) littermates. They exhibited slight increase in serum TSH (18%), with normal pituitary expression of mRNA coding for alpha-glycoprotein subunit (Cga), but reduced TSH beta-subunit mRNA (Tshb, 41%), lower intra-pituitary TSH content (24%) and increased thyroid hormone transporter MCT-8 (Slc16a2, 44%) and thyroid hormone receptor beta mRNA expression (Thrb, 39%). NBR-KO mice exhibited normal thyroxine (T4) and reduced triiodothyronine (T3) (30%), with no alterations in the intra-thyroidal content of T4 and T3 or thyroid morphological changes. Hypothalamic thyrotropin-releasing hormone (TRH) mRNA (Trh) was increased (68%), concomitant with a reduction in type 2 deiodinase mRNA (Dio2, 30%) and no changes in MCT-8 and thyroid hormone receptor mRNA expression. NBR-KO mice exhibited a 56% higher increase in serum TSH in response to an acute single intraperitoneal injection of TRH concomitant with a non-significant increase in pituitary TRH receptor (Trhr) mRNA at basal state. The phenotype of female NBR-KO mice at the hypothalamus-pituitary axis revealed alterations in pituitary and hypothalamic gene expression, associated with reduced serum T3, and higher TSH response to TRH, with apparently normal thyroid morphology and hormonal production. Thus, results confirm that neuromedin B pathways are importantly involved in secretory pathways of TSH and revealed its participation in the in vivo regulation of gene expression of TSH beta-subunit and pituitary MCT8 and Thrb and hypothalamic TRH and type 2 deiodinase. |
