| Primary Identifier | MGI:2388292 | Allele Type | Targeted |
| Gene | Dab1 | Transmission | Germline |
| Strain of Origin | 129S4/SvJaeSor | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | To generate an allele lacking phosphorylation sites, the exon encoding amino acids 23 through 69 was replaced with a construct containing a splice acceptor and a modified partial cDNA encoding the p80 splice form. The cDNA was modified to encode phenylalanines in place of the 5 tyrosines residues (185, 198, 200, 220, 232) that are potential phosphorylation sites. Phosphorylation levels were undetectable by phosphopeptide analysis of protein produced by the targeted allele in vitro. |
