| First Author | Reichlin A | Year | 2004 |
| Journal | J Exp Med | Volume | 199 |
| Issue | 6 | Pages | 855-65 |
| PubMed ID | 15024049 | Mgi Jnum | J:128768 |
| Mgi Id | MGI:3768000 | Doi | 10.1084/jem.20031140 |
| Citation | Reichlin A, et al. (2004) A B cell receptor with two Igalpha cytoplasmic domains supports development of mature but anergic B cells. J Exp Med 199(6):855-65 |
| abstractText | B cell receptor (BCR) signaling is mediated through immunoglobulin (Ig)alpha and Igbeta a membrane-bound heterodimer. Igalpha and Igbeta are redundant in their ability to support early B cell development, but their roles in mature B cells have not been defined. To examine the function of Igalpha-Igbeta in mature B cells in vivo we exchanged the cytoplasmic domain of Igalpha for the cytoplasmic domain of Igbeta by gene targeting (Igbetac-->alphac mice). Igbetac-->alphac B cells had lower levels of surface IgM and higher levels of BCR internalization than wild-type B cells. The mutant B cells were able to complete all stages of development and were long lived, but failed to differentiate into B1a cells. In addition, Igbetac-->alphac B cells showed decreased proliferative and Ca2+ responses to BCR stimulation in vitro, and were anergic to T-independent and -dependent antigens in vivo. |
