| Primary Identifier | MGI:2388336 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Lck-ZAP70-Y319F)2Achn |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| description | This transgenic founder (line 974) expresses 4-fold higher levels of transgene product compared to other transgenic lines. |
| molecularNote | The human ZAP70 gene carrying a tyrosine to phenylalanine substitution at position 319 (Y319F) was used as the transgene. The transgene is expressed in thymocytes under the control of mouse Lck promoter and contains a hemagglutinin epitope tag at the N-terminal end and a human growth hormone polyA tail. Transgene expression was verified by Western blot analysis. |
