Primary Identifier | MGI:2388344 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Rag1 |
Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The gene was disrupted by insertion (knock-in) of a GFP-neo cassette into the coding sequence. RT-PCR analysis of bone marrow RNA confirmed the absence of Rag1 gene expression in homozygous mutant animals. Instead the mutant animals produced GFP transcripts. |