| First Author | Han BY | Year | 2014 |
| Journal | Elife | Volume | 3 |
| PubMed ID | 25343476 | Mgi Jnum | J:214793 |
| Mgi Id | MGI:5604019 | Doi | 10.7554/eLife.03549 |
| Citation | Han BY, et al. (2014) Zinc finger protein Zfp335 is required for the formation of the naive T cell compartment. Elife 3 |
| abstractText | The generation of naive T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Zfp335(bloto/bloto) mice exhibit a naive T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. The effects of Zfp335(bloto) are multigenic and cannot be attributed to altered thymic selection, proliferation or Bcl2-dependent survival. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function, and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. These findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation. |
