Primary Identifier | MGI:2388380 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Sall1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The entire coding region, except for sequences encoding the 52 N-terminal amino acids, was deleted and replaced with a lacZ-neomyin resistance cassette. The mutation removes all zinc finger domains from the gene and creates a lacZ fusion protein. Absence of gene expression in homozygous mutant animals was verified by RT-PCR and in situ hybridization. Expression of the lacZ fusion protein was observed by X-gal staining of mutant embryos. |