| Primary Identifier | MGI:2388002 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Mbd2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 2 was deleted and replaced with a promoter-less beta-geo cassette via homologous recombination. Northern blot confirmed the absence of normal transcript in homozygous mutant animals. RT-PCR analysis using primers to exon 1 and exon 3 detected a faint mutant read-through product. Western blot analysis of spleen and liver nuclear extracts from homozygous animals detected a small mutant protein presumed to be the translation product from exon 1, and would lack any functional domains. |
