Primary Identifier | MGI:2388126 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ghr |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 4 and 500 bp of downstream intronic sequences were replaced with a neomycin resistance cassette via homologous recombination. Northern blot and RT-PCR analysis of liver mRNA from homozygous mutant animals failed to detect gene expression. Western blot analysis of homozygous mutant liver samples did not detect protein product. |