| Primary Identifier | MGI:2388478 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Il6st |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The Ptpn11 binding domain, encoded by sequence in exon 17, was disrupted by homologous recombination of a construct containing missense mutations at codons 757 (tyrosine to phenylalanine) and 760 (valine to alanine). Furthermore, an IRES-neo cassette was inserted into the 3' region of exon 17. A biochemical assay of liver tissue showed that the Il6st mediated signalling pathway was disrupted in mutant mice. |
