| Primary Identifier | MGI:2388486 | Allele Type | Targeted |
| Gene | Igh | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| description | The targeted IgH allele was of the a allotype. |
| molecularNote | Homologous recombination and subsequent in vitro cre-mediated excision resulted in the replacement of endogenous sequence encompassing a 3' Igh-D element (DQ52) and the Igh-J elements with a recombined antibody variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8). The B1-8 variable region was modified to contain a point mutation in codon 92, inactivating the heptameric recombination signal sequence. Flow cytometry verified cell surface expression of the mutant protein product. |
