| Primary Identifier | MGI:2429992 | Allele Type | Targeted |
| Gene | Gata1 | Transmission | Germline |
| Strain of Origin | 129S4/SvJae | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Sequence extending from the start codon in exon 2 to the EcoRI site in exon 3 was replaced with a construct containing human GATA3 cDNA sequence followed by a floxed PGK-neo cassette. Northern blot analyses of cell lines derived from mutant embryos indicated that a transcript comprised of both GATA3 and Gata1 sequence was expressed in erythroid cells (but not in myeloid cells). The presence of a protein translated from GATA3 sequence was verified by immunostaining. No Gata1 protein was detected. |
