| First Author | White JK | Year | 2013 |
| Journal | Cell | Volume | 154 |
| Issue | 2 | Pages | 452-64 |
| PubMed ID | 23870131 | Mgi Jnum | J:200082 |
| Mgi Id | MGI:5506968 | Doi | 10.1016/j.cell.2013.06.022 |
| Citation | White JK, et al. (2013) Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes. Cell 154(2):452-64 |
| abstractText | Mutations in whole organisms are powerful ways of interrogating gene function in a realistic context. We describe a program, the Sanger Institute Mouse Genetics Project, that provides a step toward the aim of knocking out all genes and screening each line for a broad range of traits. We found that hitherto unpublished genes were as likely to reveal phenotypes as known genes, suggesting that novel genes represent a rich resource for investigating the molecular basis of disease. We found many unexpected phenotypes detected only because we screened for them, emphasizing the value of screening all mutants for a wide range of traits. Haploinsufficiency and pleiotropy were both surprisingly common. Forty-two percent of genes were essential for viability, and these were less likely to have a paralog and more likely to contribute to a protein complex than other genes. Phenotypic data and more than 900 mutants are openly available for further analysis. PAPERCLIP: |
