| First Author | Frenzel K | Year | 2006 |
| Journal | Exp Cell Res | Volume | 312 |
| Issue | 15 | Pages | 2735-44 |
| PubMed ID | 16887119 | Mgi Jnum | J:112700 |
| Mgi Id | MGI:3663206 | Doi | 10.1016/j.yexcr.2006.05.004 |
| Citation | Frenzel K, et al. (2006) A functional Jak2 tyrosine kinase domain is essential for mouse development. Exp Cell Res 312(15):2735-44 |
| abstractText | Jak2 is a member of the Janus family of tyrosine kinases and is involved in cytokine signaling. As a part of a study to determine biological functions of Jak2, we used molecular modeling to identify W1038 as a residue that is critical for tyrosine kinase function. Mutation of W1038, in tandem with E1046, generates a dominant-negative form of the Jak2 protein. Mice that were engineered to express two copies of this dominant-negative Jak2 protein died in utero. Additionally, heterozygous mice expressing Jak2 with kinase activity that is moderately reduced when compared to wild-type activity appear phenotypically normal. Collectively, these data suggest that Jak2 kinase activity is essential for normal mammalian development. |
