| First Author | Liu Y | Year | 2000 |
| Journal | Curr Biol | Volume | 10 |
| Issue | 22 | Pages | 1459-62 |
| PubMed ID | 11102810 | Mgi Jnum | J:90866 |
| Mgi Id | MGI:3044894 | Doi | 10.1016/s0960-9822(00)00805-8 |
| Citation | Liu Y, et al. (2000) The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo. Curr Biol 10(22):1459-62 |
| abstractText | Mammalian telomerase is essential for the maintenance of telomere length [1-5]. Its catalytic core comprises a reverse transcriptase component (TERT) and an RNA component. While the biochemical role of mammalian TERT is well established [6-11], it is unknown whether it is sufficient for telomere-length maintenance, chromosome stability or other cellular processes. Cells from mice in which the mTert gene had been disrupted showed progressive loss of telomere DNA, a phenotype similar to cells in which the gene encoding the telomerase RNA component (mTR) has been disrupted [1,12]. On prolonged growth, mTert-deficient embryonic stem (ES) cells exhibited genomic instability, aneuploidy and telomeric fusions. ES cells heterozygous for the mTert disruption also showed telomere attrition, a phenotype that differs from heterozygous mTR cells [12]. Thus, telomere maintenance in mammals is carried out by a single, limiting TERT. |
