| First Author | Takada Y | Year | 2011 |
| Journal | Development | Volume | 138 |
| Issue | 19 | Pages | 4207-17 |
| PubMed ID | 21896631 | Mgi Jnum | J:176437 |
| Mgi Id | MGI:5291854 | Doi | 10.1242/dev.064444 |
| Citation | Takada Y, et al. (2011) HP1{gamma} links histone methylation marks to meiotic synapsis in mice. Development 138(19):4207-17 |
| abstractText | During meiosis, specific histone modifications at pericentric heterochromatin (PCH), especially histone H3 tri- and dimethylation at lysine 9 (H3K9me3 and H3K9me2, respectively), are required for proper chromosome interactions. However, the molecular mechanism by which H3K9 methylation mediates the synapsis is not yet understood. We have generated a Cbx3-deficient mouse line and performed comparative analysis on Suv39h1/h2-, G9a- and Cbx3-deficient spermatocytes. This study revealed that H3K9me2 at PCH depended on Suv39h1/h2-mediated H3K9me3 and its recognition by the Cbx3 gene product HP1gamma. We further found that centromere clustering and synapsis were commonly affected in G9a- and Cbx3-deficient spermatocytes. These genetic observations suggest that HP1gamma/G9a-dependent PCH-mediated centromere clustering is an axis for proper chromosome interactions during meiotic prophase. We propose that the role of the HP1gamma/G9a axis is to retain centromeric regions of unpaired homologous chromosomes in close alignment and facilitate progression of their pairing in early meiotic prophase. This study also reveals considerable plasticity in the interplay between different histone modifications and suggests that such stepwise and dynamic epigenetic modifications may play a pivotal role in meiosis. |
