| First Author | Lough KJ | Year | 2019 |
| Journal | Elife | Volume | 8 |
| PubMed ID | 31833472 | Mgi Jnum | J:294113 |
| Mgi Id | MGI:6445264 | Doi | 10.7554/eLife.49249 |
| Citation | Lough KJ, et al. (2019) Telophase correction refines division orientation in stratified epithelia. Elife 8:e49249 |
| abstractText | During organogenesis, precise control of spindle orientation balances proliferation and differentiation. In the developing murine epidermis, planar and perpendicular divisions yield symmetric and asymmetric fate outcomes, respectively. Classically, division axis specification involves centrosome migration and spindle rotation, events occurring early in mitosis. Here, we identify a novel orientation mechanism which corrects erroneous anaphase orientations during telophase. The directionality of reorientation correlates with the maintenance or loss of basal contact by the apical daughter. While the scaffolding protein LGN is known to determine initial spindle positioning, we show that LGN also functions during telophase to reorient oblique divisions toward perpendicular. The fidelity of telophase correction also relies on the tension-sensitive adherens junction proteins vinculin, alpha-E-catenin, and afadin. Failure of this corrective mechanism impacts tissue architecture, as persistent oblique divisions induce precocious, sustained differentiation. The division orientation plasticity provided by telophase correction may enable progenitors to adapt to local tissue needs. |
