| Primary Identifier | MGI:2445841 | Allele Type | Targeted |
| Gene | Igf2r | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The endogenous allele was released from imprint-mediated regulation by deleting the CpG rich sequences of region 2 using molecular techniques. A 3.7 kb fragment was replaced with a floxed hygro cassette which was subsequently excised in vitro via cre mediated recombination. Slot blot and RNAse protection assay analyses showed normal transcript to be produced from the targeted locus. |
