| Primary Identifier | MGI:2445993 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Hba-x-v-Ha-ras)TG.ACLed |
| Strain of Origin | FVB/NTac | Is Recombinase | false |
| Is Wild Type | false |
| description | Four lines of transgenic mice carrying 3-10 copies of the fusion gene were made. Three of the lines showed no expression of the transgene as measured by RNase protection of an antisense probe. A transgenic founder line designated "TG.AC" was continued. |
| molecularNote | The transgene consists of a mouse Hba-x promoter and an Hras1 coding sequence carrying activating mutations G12R (glycine to arginine) and A59T (alanine to threonine). The Hras1 sequence is followed by an SV40 polyadenlyation signal. RNase protection assay detected transgene expression in day 12 embryonic blood, hematopoietic fetal liver, and placenta. Low levels of transgene expression was also detected in adult bone marrow. |
