| Primary Identifier | MGI:2446391 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nthl1 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence encompassing exons 3 and 4 was replaced with a neomycin selection cassette inserted by homologous recombination. In addition to encoding the helix-hairpin-helix motif necessary for enzyme activty, the deleted region included codon 208, encoding a lysine orthologous to human lysine 212 which is a nucleophile that initiates the formation of a Schiff base ES intermediate. RT-PCR confirmed the absence of normal transcript in homozygous mutant mice. |
