Primary Identifier | MGI:2446459 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(Mylk2-Ucpd1)2Sem |
Strain of Origin | (C57BL/6 x CBA)F1 | Is Recombinase | false |
Is Wild Type | false |
description | Two transgenic founder lines were established, a low expressing line named Ucp-L and a high expressing line named Ucp-H. |
molecularNote | A 1 kb mouse Ucp1 cDNA under the control of a rat Mylk2 promoter was used for the transgene. A human ALB intron and human GAPD termination and polyadenylation signal were added to the mouse sequence. RT-PCR analysis confirmed transgene expression in skeletal muscle and no other tissues examined. |