| First Author | Urwyler-Rösselet C | Year | 2018 |
| Journal | J Invest Dermatol | PubMed ID | 29317263 |
| Mgi Jnum | J:260842 | Mgi Id | MGI:6150594 |
| Doi | 10.1016/j.jid.2017.12.031 | Citation | Urwyler-Rosselet C, et al. (2018) Keratinocyte-Specific Ablation of RIPK4 Allows Epidermal Cornification but Impairs Skin Barrier Formation. J Invest Dermatol 138(6):1268-1278 |
| abstractText | In humans, receptor-interacting protein kinase 4 (RIPK4) mutations can lead to the autosomal recessive Bartsocas-Papas and popliteal pterygium syndromes, which are characterized by severe skin defects, pterygia, as well as clefting. We show here that the epithelial fusions observed in RIPK4 full knockout (KO) mice are E-cadherin dependent, as keratinocyte-specific deletion of E-cadherin in RIPK4 full KO mice rescued the tail-to-body fusion and fusion of oral epithelia. To elucidate RIPK4 function in epidermal differentiation and development, we generated epidermis-specific RIPK4 KO mice (RIPK4(EKO)). In contrast to RIPK4 full KO epidermis, RIPK4(EKO) epidermis was normally stratified and the outside-in skin barrier in RIPK4(EKO) mice was largely intact at the trunk, in contrast to the skin covering the head and the outer end of the extremities. However, RIPK4(EKO) mice die shortly after birth due to excessive water loss because of loss of tight junction protein claudin-1 localization at the cell membrane, which results in tight junction leakiness. In contrast, mice with keratinocyte-specific RIPK4 deletion during adult life remain viable. Furthermore, our data indicate that epidermis-specific deletion of RIPK4 results in delayed keratinization and stratum corneum maturation and altered lipid organization and is thus indispensable during embryonic development for the formation of a functional inside-out epidermal barrier. |
