| First Author | Ghuwalewala S | Year | 2024 |
| Journal | Development | Volume | 151 |
| Issue | 12 | PubMed ID | 38934416 |
| Mgi Jnum | J:350921 | Mgi Id | MGI:7662818 |
| Doi | 10.1242/dev.202389 | Citation | Ghuwalewala S, et al. (2024) A transit-amplifying progenitor with biphasic behavior contributes to epidermal renewal. Development 151(12):dev202389 |
| abstractText | Transit-amplifying (TA) cells are progenitors that undergo an amplification phase followed by transition into an extinction phase. A long postulated epidermal TA progenitor with biphasic behavior has not yet been experimentally observed in vivo. Here, we identify such a TA population using clonal analysis of Aspm-CreER genetic cell-marking in mice, which uncovers contribution to both homeostasis and injury repair of adult skin. This TA population is more frequently dividing than a Dlx1-CreER-marked long-term self-renewing (e.g. stem cell) population. Newly developed generalized birth-death modeling of long-term lineage tracing data shows that both TA progenitors and stem cells display neutral competition, but only the stem cells display neutral drift. The quantitative evolution of a nascent TA cell and its direct descendants shows that TA progenitors indeed amplify the basal layer before transition and that the homeostatic TA population is mostly in extinction phase. This model will be broadly useful for analyzing progenitors whose behavior changes with their clone age. This work identifies a long-missing class of non-self-renewing biphasic epidermal TA progenitors and has broad implications for understanding tissue renewal mechanisms. |
