| Primary Identifier | MGI:2446607 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nts |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence encoding the precursor protein signal sequence was replaced with a cassette containing beta-galactosidase and neomyicin resistance genes. Transcript was undetected by Northern blot analysis of intestinal and brain samples obtained from homozygous mutant mice. Similarly, radioimmunoassay of HPLC-fractioned brain extracts and immunohistochemical analysis of brain sections indicated an absence of normal protein. Expression of the beta-galactosidase gene was not detected by staining. |
