| Primary Identifier | MGI:2446611 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Rr96 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Additional alleles engineered to lack nucleotides within the core silencer and others engineered to lack surrounding sequences showed variegated expression of Cd4. |
| molecularNote | The core region of a Cd4 cis-regulatory element that silences expression of the endogenous locus during T cell development (S4) was deleted from intron 1 via homologous recombination of a targeting construct lacking nucleotides 167 through 257. A single loxP site was left upstream of the deleted region, however analysis of a control allele indicated that the loxP site did not affect normal expression. Flow cytometric analysis of peripheral blood lymphocytes obtained from mutant mice showed no silencing of Cd4 expression. |
