| Primary Identifier | MGI:2446646 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Prnp)E11Cwe |
| Strain of Origin | B6;129S7-Prnp<tm1Cwe>/CweCnrm | Is Recombinase | false |
| Is Wild Type | false |
| description | Nuclear injections were made in zygotes carrying Prnptm1Cwe. The founder line contained approximately 70 copies of the transgenic construct. |
| molecularNote | A transgenic construct was engineered to contain murine Prnp lacking sequence encoding amino acids 32 through 121. Sequence, including that encoding the amino-terminal signal sequence, necessary for proper transport to the endoplasmic reticulum and proper processing was left intact. Transgenic expression was verified by Western blot analysis. Only after treatment with PNGase did the transgenic protein migrate a distance corresponding to the deletion, indicating in vivo glycosylation. A carboxy terminal cleavage product was also detected. The murine Prnp promoter drove expression of similar transgenes (lines 11 and 12) the forebrain and cerebellum. Transgenic transcript was identified in all layers of the cerebellum except for Purkinje cells. |
