| Primary Identifier | MGI:2447086 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Prnp-PG14)1Dah |
| Strain of Origin | (C57BL/6J x CBA/J)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Five transgenic founder were created. Founder A1 carries 28 copies of the transgene, founder A2 carries 5 copies, founder A3 carries 33 copies, founder B carries 2 copies, and founder C carries 4 copies. Founder A1 was unable to breed. |
| molecularNote | The transgene consists of 12 kb of the mouse Prnp promoter region, which includes intron 1, and a mutant Prnp cDNA containing 9 octapeptide insertions rich in glycine and proline residues. This mutation is found in human prion diseases. The transgene also includes an epitope tag for monoclonal antibody 3F4. Transgene expression was verified by Western blot analysis of brain homogenates from transgenic animals. |
