| First Author | Pivniouk V | Year | 1998 |
| Journal | Cell | Volume | 94 |
| Issue | 2 | Pages | 229-38 |
| PubMed ID | 9695951 | Mgi Jnum | J:48828 |
| Mgi Id | MGI:1275861 | Doi | 10.1016/s0092-8674(00)81422-1 |
| Citation | Pivniouk V, et al. (1998) Impaired viability and profound block in thymocyte development in mice lacking the adaptor protein SLP-76. Cell 94(2):229-38 |
| abstractText | The adaptor protein SLP-76 is expressed in T lymphocytes and myeloid cells and is a substrate for ZAP-70 and Syk. We generated a SLP-76 null mutation in mice by homologous recombination in embryonic stem cells to evaluate the role of SLP-76 in T cell development and activation. SLP-76-deficient mice exhibited subcutaneous and intraperitoneal hemorrhaging and impaired viability. Analysis of lymphoid cells revealed a profound block in thymic development with absence of double-positive CD4+8+ thymocytes and of peripheral T cells. This block could not be overcome by in vivo treatment with anti-CD3. V-D-J rearrangement of the TCRbeta locus was not obviously affected. B cell development was normal. These results indicate that SLP-76 collects all pre-TCR signals that drive the development and expansion of double-positive thymocytes. |
