| First Author | Rouleau M | Year | 1999 |
| Journal | J Immunol | Volume | 163 |
| Issue | 3 | Pages | 1420-7 |
| PubMed ID | 10415042 | Mgi Jnum | J:56993 |
| Mgi Id | MGI:1343012 | Doi | 10.4049/jimmunol.163.3.1420 |
| Citation | Rouleau M, et al. (1999) IL-10 transgenic mice present a defect in T cell development reminiscent of SCID patients. J Immunol 163(3):1420-7 |
| abstractText | To analyze the effect of IL-10 overexpressed by APCs as observed in some SCID patients, we have expressed the human IL-10 cDNA under the control of the murine MHC class II promoter in transgenic mice. Similar to SCID patients, these mice presented a defect in T cell maturation characterized by a rapid thymic aplasia that started after birth. The blockage in T cell maturation was strictly restricted to TCR-alpha beta T cells as the absolute number of thymic dendritic, TCR-gamma delta and NK1.1 T cells were equivalent to control littermates. Crossing IL-10 transgenic mice with TCR transgenic mice or treatment with staphylococcal enterotoxin B showed that the defect was not related to the impairment of positive or negative selection. However, repopulating of IL-10 transgenic mouse-fetal thymic organ culture with different stages of triple negative T cells isolated from control mice showed that the blockage occurred specifically at the pre-T cell stage and was reverted by treatment with blocking anti-IL-10 mAbs. These results demonstrate that IL-10 regulates T cell maturation and that dysregulation of IL-10 expression can lead to severe T cell immunodeficiency. |
