| Primary Identifier | MGI:2447580 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(H2-K-BCL2)1Josd |
| Strain of Origin | (C57BL/6 x C3H)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Ten transgenic founders were created. Transgenic founders 1038, 1043, and 1053 were used for analysis. All founders tested exhibit similar patterns of transgene expression. |
| molecularNote | A human BCL2 cDNA under regulatory control of the H2-K promoter was used for the transgene. The H2-K promoter was used to drive transgene expression in hematopoietic stem cells. Northern blot analysis detected strong transgene expression in kidney, spleen, thymus, and lung. Weak expression was observed in the liver. Furthermore, Western blot analysis showed transgene expression in lymph nodes, heart, spleen, thymus, liver, and lungs. |
