| Primary Identifier | MGI:2447708 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Smarcb1 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked neomycin selection cassette was inserted into intron 1 and a single loxP site in the opposite orientation was inserted 0.8 kb upstream of exon 1. The neomycin selection cassette was removed by transient Cre expression in ES cells, leaving oppositely oriented loxP sites flanking exon 1 in the final allele. Upon Cre-mediated recombination, exon 1 and flanking sequences are inverted in orientation with respect to the rest of the gene. |
