| Primary Identifier | MGI:2447712 | Allele Type | Transgenic |
| Attribute String | Constitutively active, Inserted expressed sequence | Gene | Tg(BLG-STAT5A)15Itb |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| description | This is transgenic line BGL/STAT5ca-15. In addition to line 15, J:80588 shows data for lines BGL/STAT5ca-8, and BGL/STAT5ca-19. Three other transgenic lines were created (BGL/STAT5ca-7, -10, and -15). Lines BGL/STAT5ca-8 and -15 carry 10 copies of the integrated transgene, line BGL/STAT5ca-7 carries 5 copies, line BGL/STAT5ca-10 carries 6 copies, and line BGL/STAT5ca-19 carries 3 copies. |
| molecularNote | The transgene consists of a beta-lactoglobulin promoter, active in mammary glands, and a fusion gene comprised of amino acids 1-750 of the sheep STAT5A gene, the terminal transactivation domain (amino acids 677-847) of human STAT6, and the kinase domain (amino acids 757-1129) of mouse Jak2. The resulting protein is a constitutively active STAT5a molecule with self-activating properties. Transgene expression was confirmed by Western blot and RT-PCR analysis of mammary glands from transgenic animals at day 6 of lactation. |
