| Primary Identifier | MGI:2447728 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Lta |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The entire Lta gene and the majority of the Tnf gene (to codon 113 in exon 4) were replaced by a neomycin selection cassette inserted by homologous recombination. No transcript or truncated protein was detected by a variety of assays including Northern blot analysis of concanavalin A stimulated splenocytes and ELISA of macrophage colony supernatants. Tnf activity was undetected in LPS stimulated homozygous mutant mice. |
