| Primary Identifier | MGI:2447845 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(RHO-SCA7)R7EJman |
| Strain of Origin | C57BL/6 x SJL | Is Recombinase | false |
| Is Wild Type | false |
| description | Transgenic lines A, B, C, D, and F were constructed. Line A carries 10-50 copies of the integrated transgene and line F carries 1-5 copies. Lines A and F express the highest amount of transgene product and were used for analysis. |
| molecularNote | The transgene consists of a human rhodopsin promoter (containing the RER enhancer element), the full-length mutant human Sca7 gene carrying a 90 glutamine repeat expansion, and an SV40 intron and polyadenylation sequence. Transgene expression was observed in rod cells by immunofluorescence, and in retinal extracts by Western blot. |
