| Primary Identifier | MGI:2447853 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Pcp2-ATXN1*30Q)2Horr |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| description | Seven transgenic lines were created carrying between 5-200 copies of the integrated transgene. Lines A02, A06.1, and A06.2 exhibited the highest levels of transgene mRNA expression at 50- to 100-fold compared to the endogenous gene. |
| molecularNote | The transgene consists of a mouse Pcp2 promoter and a human wild-type SCA1 cDNA sequence with 30 CAG repeats. The 835 bp fragment of mouse Pcp2 regulatory sequence include exon 1, intron 1, and part of exon 2 of Pcp2. The Pcp2 promoter targets transgene expression to Purkinje cells. Transgene expression was confirmed by Northern blot and Western blot analysis of cerebellar extracts. |
