| Primary Identifier | MGI:2447531 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Igh-Pim1)1Brn |
| Strain of Origin | (CBA x C57BL/6LiA)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Transgenic lines ppG66 and ppG64 carrying 2-4 copies of the transgene were created. Both lines exhibit similar transgene expression patterns. The ppG64 line showed some transgene instability. Subline ppG64a resembles the original founder whereas subline ppG64b lacks one founder-specific band on the Southern blot. |
| molecularNote | The transgene consists of a duplicated Igh enhancer (Emu), the Pim1 gene, and a mouse Moloney leukemia virus LTR. Transgene expression was confirmed by Northern and Western blot analysis. High expression of Pim1 mRNA was observed in the thymus, bone marrow, and spleen, but not in testis. Pim1 protein expression is observed in both B and T lymphocytes. |
